Review Paper – Fundamental Developments in IR Spectroscopic Imaging for Biomedical Applications

Fundamental developments in infrared spectroscopic imaging for biomedical applications
Michael Pilling and Peter Gardner
Chem. Soc. Rev., 2016,45, 1935-1957
DOI: 10.1039/C5CS00846H

Abstract

Infrared chemical imaging is a rapidly emerging field with new advances in instrumentation, data acquisition and data analysis. These developments have had significant impact in biomedical applications and numerous studies have now shown that this technology offers great promise for the improved diagnosis of the diseased state. Relying on purely biochemical signatures rather than contrast from exogenous dyes and stains, infrared chemical imaging has the potential to revolutionise histopathology for improved disease diagnosis. In this review we discuss the recent advances in infrared spectroscopic imaging specifically related to spectral histopathology (SHP) and consider the current state of the field. Finally we consider the practical application of SHP for disease diagnosis and consider potential barriers to clinical translation highlighting current directions and the future outlook.

Graphical abstract: Fundamental developments in infrared spectroscopic imaging for biomedical applications