FTIR, Raman and SIMS Imaging for Lipidomic Analysis of Cellular Systems
Deadline 17 November 2017
The role of lipid metabolism in a number of cellular processes including (i) stem cell differentiation, (ii) drugcell interactions and (iii) epithelial/adipocyte cell interactions, is generally poorly understood. For example it has been recently shown that PC3 cells when co-cultured with adipocyte cells, sequestrate omega-6 lipids and their metabolites which subsequently stimulate cell migration and whilst promoting proliferation . Similarly lipids have been shown to play a key role in the differentiation of stem-cells, and recent investigations using FTIR have shown that lipid signatures may indicate early signs of differentiation . These fundamental cell processes, mediated by lipids, are currently a major focus of research.
Probing the secondary effects of Tp53 and BRCA gene mutations upon cellular physiology using advanced analytical techniques.
Prof R Edmondson, Prof P Gardner, Dr N Lockyer, Dr J Denbigh
Deadline: 17 November 2017
Cancer is a disease of DNA in which genomic events allow the cell to develop the autonomy, increased proliferation and other fundamental hallmarks of the disease. This process is often initiated by mutation of one or two key driver genes. Understanding the effects of these driver mutations is crucial in order to not only improve our understanding of the disease process but also to develop new screening and detection methods for cancer. In this exciting PhD the student will develop a novel cell model using primary human tissue to replicate the earliest phases in the development of high grade serous cancer, the commonest and most deadly pelvic cancer. The model will be created using fallopian tube epithelial cells which will be cultured ex vivo. Tp53 and BRCA1 genes will then be silenced using Crispr technology.