Dr Alex Henderson

Alex Henderson

Senior Experimental Officer

Honorary Lecturer in Cheminformatics

Honorary Lecturer in Analytical Science


alex.henderson @ manchester.ac.uk



Alex Henderson
ORCID Account

Research

My research interests lie in data reduction, working mainly with the results from IR (infra red spectroscopy) and ToF-SIMS (time of flight secondary ion mass spectrometry) experiments in cell chemistry.

Chemometric and cheminformatic approaches are applied to spectra, 2D chemical images and 3D chemical hypercubes to understand the complex chemistries involved in cell structure, metabolism and drug interaction. Work carried out in parallel aims to develop visualisation tools for chemists and biologists working in this area.

In addition to my work in the FTIR field I also work on ToFSIMS data of tissue and inorganic materials as a member of John Vickerman’s research group

Teaching

I recently taught Instrumentation for Separation Science which was a module of our Analytical and Separation Science Masters programme. My lectures covered the interpretation of mass spectrometric data.

Previously I taught Database Design and Programming on the School of Chemistry’s Cheminformatics Masters programme.

Commercial

I am a director of SurfaceSpectra Ltd. and also write their software.

Other

Recognised UK National Expert

International Standard Organisation (ISO), Technical Committee 201 Surface Chemical Analysis.

Conference organising committees

  • 59th IUVSTA Workshop Surface Chemical Analysis – Improving Data Interpretation by Multivariate and Informatics Techniques
    Island of Trinidad, 2010 (Scientific Committee)
  • The 6th International SPEC conference
    Manchester 2010 (Local Organising Committee)
  • The 15th International Conference on Secondary Ion Mass Spectrometry (SIMS XV)
    Manchester 2005 (Local Organising Committee)

Links

Here are links to articles I have found particularly interesting/useful.

Publications

46 Infrared spectral histopathology using haematoxylin and eosin (H&E) stained glass slides: a major step forward towards clinical translation
Michael J. Pilling, Alex Henderson, Jonathan H. Shanks, Michael D. Brown, Noel W. Clarke and Peter Gardner
Analyst (2017)
45 FTIR imaging of the molecular burden around Aβ deposits in an early-stage 3-Tg-APP-PSP1-TAU mouse model of Alzheimer’s disease
Artur Dawid Surowka, Michael Pilling, Alex Henderson, Herve Boutin, Lidan Christie, Magdalena Szczerbowska-Boruchowska and Peter Gardner
Analyst 142 (2017) 156-168
44 High throughput Quantum Cascade Laser (QCL) spectral histopathology : a practical approach towards clinical translation
Michael J Pilling, Peter Gardner, Alex Henderson, Michael D Brown, Benjamin Bird and Noel William Clarke
Faraday Discuss. 187 (2016) 135-154
43 Evaluation of biomolecular distributions in rat brain tissues by means of ToF-SIMS using a continuous beam of Ar clusters
Shusuke Nakano, Yuta Yokoyama, Satoka Aoyagi, Naoyuki Himi, John S. Fletcher, Nicholas P. Lockyer, Alex Henderson and John C. Vickerman
Biointerphases 11 (2016) 02A307
42 Mass spectrometric imaging of brain tissue by time-of-flight secondary ion mass spectrometry – How do polyatomic primary beams C60+, Ar2000+, water-doped Ar2000+ and (H2O)6000+ compare?
Irma Berrueta Razo, Sadia Sheraz (née Rabbani), Alex Henderson, Nicholas P. Lockyer and John C. Vickerman
Rapid Commun. Mass Spectrom. 29 (2015) 1851–1862
41 Enhanced FTIR benchtop imaging of single biological cells
Caryn Hughes, Alex Henderson, Mustafa Kansiz, Konrad Dorling, Melody Jimenez-Hernandez, Michael D Brown, Noel William Clarke and Peter Gardner
Analyst 140 (2015) 2080–2085
40 Spatiotemporal lipid profiling during early embryo development of Xenopus laevis using dynamic Time-of-Flight Secondary Ion Mass Spectrometry (ToF-SIMS) Imaging
Hua Tian, John S. Fletcher, Raphael Thuret, Alex Henderson, Nancy Papalopulu, John C. Vickerman and Nicholas P. Lockyer
Journal of Lipid Research 55 (2014) 1970-1980
39 Comparing C60+ and (H2O)n+ clusters for mouse brain tissue analysis
Irma Berrueta Razo, Sadia Sheraz, Alex Henderson, Nicholas P. Lockyer and John C. Vickerman
Surf. Interface Anal. 46 (2014) 136–139
38 Assessing the challenges of Fourier transform infrared spectroscopic analysis of blood serum
Caryn Hughes, Michael Brown, Graeme Clemens, Alex Henderson, Geraldine Monjardez, Noel W. Clarke, Peter Gardner
Journal of Biophotonics 7 (2014) 180–188
37 Multivariate Analysis of SIMS Spectra
Alex Henderson
in “TOF-SIMS: Materials Analysis by Mass Spectrometry”, 2nd edition, Eds. J.C. Vickerman and D. Briggs, SurfaceSpectra, Manchester and IM Publications, Chichester (2013) 449-484
36 Peptide structural analysis using continuous Ar cluster and C60 ion beams
Satoka Aoyagi, John S. Fletcher, Sadia Sheraz (Rabbani), Tomoko Kawashima, Irma Berrueta Razo, Alex Henderson, Nicholas P. Lockyer, John C. Vickerman
Analytical and Bioanalytical Chemistry 405 (2013) 6621-6628
35 The inherent problem of transflection-mode infrared spectroscopic microscopy and the ramifications for biomedical single point and imaging applications
Paul Bassan, Joe Lee, Ashwin Sachdeva, Juliana Pissardini, Konrad M. Dorling, John S. Fletcher, Alex Henderson and Peter Gardner
Analyst 138 (2013) 144-157
34 ToF-SIMS as a tool for metabolic profiling small biomolecules in cancer systems
Helen L. Kotze, Emily G. Armitage, John S. Fletcher, Alex Henderson, Kaye J. Williams, Nicholas P. Lockyer and John C. Vickerman
Surf. Interface Anal. 45 (2013) 277-281
33 SIMS informatics
Alex Henderson, Jimmy D. Moore and John C. Vickerman
Surf. Interface Anal. 45 (2013) 471-474
32 Peak picking as a pre-processing technique for imaging time of flight secondary ion mass spectrometry
Jimmy D. Moore, Alex Henderson, John S. Fletcher, Nicholas P. Lockyer and John C. Vickerman
Surf. Interface Anal. 45 (2013) 461-465
31 Time-of-flight SIMS as a novel approach to unlocking the hypoxic properties of cancer
Emily G Armitage, Helen L Kotze, John S Fletcher, Alex Henderson, Kaye J Williams, Nicholas P Lockyer and John C Vickerman
Surf. Interface Anal. 45 (2013) 282-285
30 FTIR microscopy of biological cells and tissue: data analysis using resonant Mie scattering (RMieS) EMSC algorithm
Paul Bassan, Ashwin Sachdeva, Achim Kohler, Caryn Hughes, Alex Henderson, Jonathan Boyle, Jonathan H. Shanks, Michael Brown, Noel W. Clarke and Peter Gardner
Analyst 137 (2012) 1370-1377
29 Interactive spatio-spectral analysis of three-dimensional mass-spectral (3DxMS) chemical images.
Stephen E. Reichenbach, Xue Tian, Robert Lindquist, Qingping Tao, Alex Henderson and John C. Vickerman
Surf. Interface Anal. 43 (2011) 529-534
28 Three-dimensional mass spectral imaging of HeLa-M cells – sample preparation, data interpretation and visualisation.
John S. Fletcher, Sadia Rabbani, Alex Henderson, Nicholas P. Lockyer, John C. Vickerman
Rapid Commun. Mass Spectrom. 25 (2011) 925-932
27 Phenotypic profiling of keloid scars using FT-IR microspectroscopy reveals a unique spectral signature
Katherine A. Hollywood, Marlies Maatje, Iqbal T. Shadi, Alex Henderson, Duncan Angus McGrouther, Royston Goodacre and Ardeshir Bayat
Archives of Dermatological Research 302 (2010) 705-715
26 Factors influencing the discrimination and classification of prostate cancer cell lines by FTIR microspectroscopy.
T. J. Harvey, E. Gazi, A. Henderson, R. D. Snook, N. W. Clarke, M. Brown and P. Gardner
Analyst 134 (2009) 1083-1091
25 Classification and Cluster Analysis of Complex Time-of-Flight Secondary Ion Mass Spectrometry for Biological Samples
Stephen E. Reichenbach, Xue Tian, Qingping Tao, and Alex Henderson
International Conference on Bioinformatics, Computational Biology, Genomics and Chemoinformatics (BCBGC-09) (2009) 78
24 Visualization and analysis of large three-dimensional hyperspectral images
Stephen E. Reichenbach, Xue Tian, Robert Lindquist, Qingping Tao, Alex Henderson, and John C. Vickerman
Proceedings of SPIE 7341 (2009) 734108
23 A comparison of PCA and MAF for ToF-SIMS image interpretation.
Alex Henderson, John S. Fletcher, John C. Vickerman
Surf. Interface Anal. 41 (2009) 666-674
22 Explanatory multivariate analysis of ToF-SIMS spectra for the discrimination of bacterial isolates.
Seetharaman Vaidyanathan, John S. Fletcher, Roger M. Jarvis, Alex Henderson, Nicholas P. Lockyer, Royston Goodacre and John C. Vickerman
Analyst 134 (2009) 2352-2360
21 Classification of fixed urological cells using Raman tweezers.
Harvey, Tim J.; Hughes, Caryn; Ward, Andrew D.; Faria, Elsa Correia; Henderson, Alex; Clarke, Noel W.; Brown, Mick D.; Snook, Richard D.; Gardner, Peter.
J. Biophotonics 2 (2009) 47-69
20 Efficient encoding and rapid decoding for interactive visualization of large three-dimensional hyperspectral chemical images.
Reichenbach, Stephen E.; Henderson, Alex; Lindquist, Robert; Tao, Qingping.
Rapid Commun. Mass Spectrom. 23 (2009) 1229-1233
19 Uncovering new challenges in bio-analysis with ToF-SIMS.
John S. Fletcher, Alex Henderson, Gregory X. Biddulph, Seetharaman Vaidyanathan, Nicholas P. Lockyer and John C. Vickerman
Applied Surface Science 255 (2008) 1264-1270
18 Exploratory analysis of TOF-SIMS data from biological surfaces.
Seetharaman Vaidyanathan, John S. Fletcher, Alex Henderson, Nicholas P. Lockyer and John C. Vickerman
Applied Surface Science 255 (2008) 1599-1602
17 A New Dynamic in Mass Spectral Imaging of Single Biological Cells.
John S. Fletcher, Sadia Rabbani, Alex Henderson, Paul Blenkinsopp, Steve P. Thompson, Nicholas P. Lockyer, and John C. Vickerman
Analytical Chemistry 80 (2008) 9058-9064
16 Spectral discrimination of live prostate and bladder cancer cell lines using Raman optical tweezers
Tim J. Harvey, Elsa Correia Faria, Alex Henderson, Ehsan Gazi, Andrew D. Ward, Noel W. Clarke, Michael D. Brown, Richard D. Snook and Peter Gardner
Journal of Biomedical Optics 13 (2008) 064004
15 Discrimination of prostate cancer cells by reflection mode FTIR photoacoustic spectroscopy.
Harvey, Tim J.; Henderson, Alex; Gazi, Ehsan; Clarke, Noel W.; Brown, Mick; Faria, Elsa Correia; Snook, Richard D.; Gardner, Peter.
Analyst 132 (2007) 292-295
14 The Static SIMS Library version 4
J.C. Vickerman, D. Briggs and A. Henderson
SurfaceSpectra, Manchester (2006) CDROM
13 Polyethylene terephthalate (PET) bulk film analysis using C60 +, Au3 +, and Au+ primary ion beams.
Conlan, X. A.; Gilmore, I. S.; Henderson, A.; Lockyer, N. P.; Vickerman, J. C.
Applied Surface Science 252 (2006) 6562-6565
12 ToF-SIMS studies of Bacillus using multivariate analysis with possible identification and taxonomic applications.
Thompson, C. E.; Ellis, J.; Fletcher, J. S.; Goodacre, R.; Henderson, A.; Lockyer, N. P.; Vickerman, J. C.
Applied Surface Science 252 (2006) 6719-6722
11 Rapid discrimination of the causal agents of urinary tract infection using ToF-SIMS with chemometric cluster analysis.
Fletcher, John S.; Henderson, Alexander; Jarvis, Roger M.; Lockyer, Nicholas P.; Vickerman, John C.; Goodacre, Royston.
Applied Surface Science 252 (2006) 6869-6874
10 Secondary Ion Mass Spectrometry, SIMS XV. (Proceedings of the Fifteenth International Conference held in UK 12-16 September 2005.) [In: Appl. Surf. Sci., 2006; 252(19)].
Vickerman, John C.; Gilmore, Ian S.; Dowsett, Mark G.; Henderson, Alex; Editors.
Applied Surface Science 252 (2006) 922 pp.
9 Summary of ISO/TC 201 standard: XXII. ISO 22048: 2004 – surface chemical analysis – information format for static secondary ion mass spectrometry.
Gilmore, I. S.; Seah, M. P.; Henderson, A.
Surface and Interface Analysis 36 (2004) 1642-1644
8 ToF-SIMS Studies of Sulfuric Acid Hydrate Films.
Fletcher, John S.; Henderson, Alex; Horn, Andrew B.; Vickerman, John C.
Journal of Physical Chemistry B 108 (2004) 5960-5966
7 Identification of surface molecular hydrates on solid sulfuric acid films.
Couling, Suzanne B.; Nash, Karen L.; Fletcher, John; Henderson, Alex; Vickerman, John C.; Horn, Andrew B.
Journal of the American Chemical Society 125 (2003) 13038-13039
6 The role of surface molecular hydrates in the heterogeneous interaction of NH3 with sulfuric acid monohydrate.
Nash, Karen L.; Sayer, Robert M.; Couling, Suzanne B.; Fletcher, John; Henderson, Alex; Vickerman, John C.; Horn, Andrew B.
Physical Chemistry Chemical Physics 5 (2003) 5101-5107
5 The Static SIMS Library version 3
J.C. Vickerman, D. Briggs and A. Henderson
SurfaceSpectra, Manchester (2002) CDROM
4 NeuroSpectraNet – a self-organising neural network mechanism for interpretation of complex SSIMS spectra.
Sanni, O. D.; Henderson, A.; Briggs, D.; Vickerman, J. C.
Secondary Ion Mass Spectrometry, SIMS XII, Proceedings of the International Conference on Secondary Ion Mass Spectrometry, 12th, Brussels, Belgium, Sept. 5-10, 1999 (2000) 805-808
3 The Static SIMS Library version 2
J.C. Vickerman, D. Briggs and A. Henderson
SurfaceSpectra, Manchester (1999) pp1200 & CDROM
2 The Static SIMS Library
J.C. Vickerman, D. Briggs and A. Henderson
SurfaceSpectra, Manchester (1997) pp1000 & CDROM
1 The effect of corona treatments on the hygral expansion of wool worsted fabrics.
Carr, C. M.; Dodd, K. J.; Connors, M. A.; Henderson, A.; Vickerman, J.
Journal of the Society of Dyers and Colourists 110 (1994) 383-6

Acknowledged in…

Here are links to articles in which I have been acknowledged.

Attenuated Total Reflection Fourier Transform Infrared (ATR-FTIR) spectral discrimination of brain tumour severity from serum samples
James R. Hands, Konrad M. Dorling, Peter Abel, Katherine M. Ashton, Andrew Brodbelt, Charles Davis, Timothy Dawson, Michael D. Jenkinson, Robert W. Lea, Carol Walker and Matthew J. Baker
Journal of Biophotonics 7(3-4) (2014) 189–199
Effect of Substrate Choice and Tissue Type on Tissue Preparation for Spectral Histopathology by Raman Microspectroscopy
Leanne M Fullwood, David M Griffiths, Katherine Ashton, Timothy Dawson, Robert W Lea, Charles Davis, Franck Bonnier, Hugh Byrne and Matthew J Baker
Analyst 139 (2014) 446-454
Single Cell Lipidomics: Characterizing and Imaging Lipids on the Surface of Individual Aplysia Californica Neurons with Cluster SIMS
Melissa K. Passarelli, Andrew G. Ewing and Nicholas Winograd
Analytical Chemistry 85(4) (2013) 2231–2238
Imaging of metabolites using secondary ion mass spectrometry
Emily G. Armitage, Helen L. Kotze, Nicholas P. Lockyer
Metabolomics 9 (2013) s102-s109
ToF-SIMS PC-DFA analysis of prostate cancer cell lines.
M.J. Baker, E. Gazi, M.D. Brown, N.W. Clarke, J.C. Vickerman and N.P. Lockyer
Appl. Surf. Sci. 255 (2008) 1084-1087
TOF-SIMS investigation of Streptomyces coelicolor, a mycelial bacterium.
Seetharaman Vaidyanathan, John S. Fletcher, Nicholas P. Lockyer and John C. Vickerman
Appl. Surf. Sci. 255 (2008) 922-925
Subsurface Biomolecular Imaging of Streptomyces coelicolor Using Secondary Ion Mass Spectrometry.
Seetharaman Vaidyanathan, John S. Fletcher, Roy Goodacre, Nicholas P. Lockyer, Jason Micklefield, and John C. Vickerman
Analytical Chemistry 80 (2008) 1942-1951
Depth Profiling Brain Tissue Sections with a 40 keV C60+ Primary Ion Beam.
Emrys A. Jones, Nicholas P. Lockyer, John C. Vickerman
Analytical Chemistry 80 (2008) 2125-2132
TOF-SIMS 3D Biomolecular Imaging of Xenopus laevis Oocytes Using Buckminsterfullerene (C60) Primary Ions.
John S. Fletcher, Nicholas P. Lockyer, Seetharaman Vaidyanathan, John C. Vickerman
Analytical Chemistry 79 (2007) 2199-2206

Named on…

Here are links to grants on which I have been funded.

2014 Clinical Infrared and Raman Spectroscopy Network (CLIRSPEC)
Peter Gardner (PI), Matthew Baker (UCLAN)
EPSRC £188k
February 2014 January 2017
2013 Towards disease diagnosis through spectrochemical imaging of tissue architecture.
Peter Gardner (PI)
EPSRC £324k
August 2013 July 2016
2013 Automated breast cancer screening: An interdisciplinary pilot study bringing together chemical imaging, cancer pathology and machine learning to produce a next generation healthcare technology.
Peter Gardner (PI), Jonathan Shapiro, Kaye Williams and Michael Lisanti
University of Manchester Research Institute (UMRI) – Pump Priming Programme £26k
2013 Application of single cell metabolite profiling to optimisation of stem cell bioprocessing
A.J. Dickson (PI), K.E. Cosgrove, M.J. Dunne, R. Goodacre, N.P. Lockyer
BBSRC £564k
2011 MaDAM into Sustainable Service (MiSS)
Lorraine Beard, PI (Head of Information Services, JRUL), Project Manager June Finch
JISC £245k + £730k institutional support
October 2011 March 2013 (extension of the MaDAM project)
2011 Infrared Imaging for Diagnosis and Prediction of the Biopotental of Low and Intermediate Risk Prostate Cancer
Gardner (PI), Brown, Clarke
EPSRC £476k
April 2011 March 2014
2011 MIB CDFA – Application of novel imaging technologies towards quantitative analytical phenotyping of single cells
Flitsch (PI), Goddard, Vickerman, Bayat, Westerhoff, Yuan, Webb, Gardner, Fielden, Goodacre, Lockyer, Fletcher, Blanch
EPSRC £201k
January 2011 January 2012
2009 A new dynamic in mass spectral imaging for biological systems
Vickerman (PI), Lockyer, Fletcher
EPSRC £740k
June 2009 May 2012
2009 A combined micro fluidic single cell SRIR microscopy stage for use at Diamond
Gardner (PI), Cinque, Fielden, Goddard
EPSRC £170k
January 2009 December 2012
2007 Enhancing new developments in ToF-SIMS through researcher exchanges
Vickerman, Lockyer, Webb (Surrey)
EPSRC Collaborating for Success Through People Award £110k
2005 New Developments in ToF-SIMS Surface Mass Spectrometry with ATR-IR Spectroscopy
Vickerman (PI), Lockyer, Gardner, Horn, Goodacre; Webb and Kirkby (Surrey).
EPSRC Critical Mass Award £2M
October 2005 Sept 2009
2004 A High Resolution ToF-SIMS Microscope for Biology
Vickerman (PI), Lockyer, Goodacre and Kell.
BBSRC £428k
August 2004 December 2007
2000 Development of ToF-SIMS instrumentation and methodology for chemical microscopy of cellular systems
Vickerman, Lockyer and Stephens
EPSRC Life Sciences Interface £312k
June 2000 June 2003
1996 Wiley Static SIMS Library – Ongoing funding for updates £13,000/year
1994 Extension to Static SIMS Library Grant July 1994 to May 1995 – John Wiley & Sons, £17,000
1992 Support from John Wiley & Sons for the launch costs (>£50k) of an International Static SIMS Database.

Other links…

LinkedIn profile
http://www.linkedin.com/in/alexhendersoninmanchester

Mass Spectrometry @ Manchester
http://massspecmanchester.org/

SimonCGI
http://www.simoncgi.com/

John Vickerman’s Research Group
Surface Analysis Research Centre

CLIRSPEC Network
http://clirspec.org/

Claire Tindale
http://www.axisweb.org/p/clairetindale/

Paul Bassan’s company: London Spectroscopy
http://www.london-spectroscopy.com/

 

 

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