Dr Paul Bassan

Paul Bassan

Research Associate


paul.bassan @ manchester.ac.uk


Paul Bassan

  • PhD on Biomedical Fourier transform infared (FTIR) spectroscopy of highly scattering samples: Understanding and correcting resonant Mie scattering (RMieS), University of Manchester, UK. 2011
  • MSc in Medical Physics, University of Manchester, UK. 2007
  • BSc Physics, University of Warwick, UK. 2006
  • CEO, London Spectroscopy Ltd.

Paul Bassan received his B.Sc. in Physics at the University of Warwick U.K. in 2006 and then went on to receive an M.Sc. in Medical Physics at the University of Manchester U.K. in 2007. In October 2007 he joined the Gardner group at the University of Manchester and is currently researching the fundamental principles of mid-IR light scattering (namely Mie scattering) in single cell and tissue samples as used in FTIR spectroscopy. Eventual aims are to produce a correction algorithm which recovers the pure absorbance spectra of highly scattering samples. A preliminary version called the resonant Mie scattering extended multiplicative signal correction (RMieS-EMSC) has been written (in collaboration with Achim Kohler and Harald Martens of Nofima Mat, Norway) and distributed free of charge to academic users. In the future he plans to apply the RMieS-EMSC to FTIR imaging data with the use of parallel computing using graphics processing unit (GPU) computing.

Areas of experience

  • Matlabprogramming
    • Writing algorithms
    • GPU Processing
  • FTIR of highly scattering samples
    • Single biological cells
    • Resonant Mie scattering (RMieS-EMSC)

Books

  1. Paul Bassan, and Peter Gardner, Scattering in Biomedical Infrared Spectroscopy, in Biomedical Applications of Synchrotron Infrared Microspectroscopy, The Royal Society of Chemistry. p. 260-276.

Publications

17 Comparison of transmission and transflectance mode FTIR imaging of biological tissue
Michael J. Pilling, Paul Bassan and Peter Gardner
Analyst 140 (2015) 2383-2392
16 Using Fourier transform IR spectroscopy to analyze biological materials
Matthew J Baker, Júlio Trevisan, Paul Bassan, Rohit Bhargava, Holly J Butler, Konrad M Dorling, Peter R Fielden, Simon W Fogarty, Nigel J Fullwood, Kelly A Heys, Caryn Hughes, Peter Lasch, Pierre L Martin-Hirsch, Blessing Obinaju, Ganesh D Sockalingum, Josep Sulé-Suso, Rebecca J Strong, Michael J Walsh, Bayden R Wood, Peter Gardner & Francis L Martin
Nature Protocols 9 (2014) 1771–1791
15
Open Access
Large scale infrared imaging of tissue micro arrays (TMAs) using a tunable Quantum Cascade Laser (QCL) based microscope
Paul Bassan, Miles J. Weida, Jeremy Rowlette and Peter Gardner
Analyst 139 (2014) 3856-3859
14 Automated high-throughput assessment of prostate biopsy tissue using infrared spectroscopic chemical imaging
Paul Bassan, Ashwin Sachdeva, Jonathan H. Shanks, Mick D. Brown, Noel W. Clarke and Peter Gardner
Proc. SPIE 9041, Medical Imaging 2014: Digital Pathology 9041 (2014) 90410D-90410D-10
13 Transmission FTIR chemical imaging on glass substrates: applications in infrared spectral histopathology
Paul Bassan, Joe Mellor, Jonathan Shapiro, Kaye J. Williams, Michael Lisanti and Peter Gardner
Anal. Chem. 86(3) (2014) 1648–1653
12
Open Access
Whole organ cross-section chemical imaging using label-free mega-mosaic FTIR microscopy
Paul Bassan, Ashwin Sachdeva, Jonathan H. Shanks, Mick D. Brown, Noel W. Clarke and Peter Gardner
Analyst 138 (2013) 7066-7069
11 The action of all-trans-retinoic acid (ATRA) and synthetic retinoid analogues (EC19 and EC23) on human pluripotent stem cells differentiation investigated using single cell infrared microspectroscopy.
Graeme Clemens, Kevin R. Flower, Andrew P. Henderson, Andrew Whiting, Stefan A. Przyborski, Melody Jimenez-Hernandez, Francis Ball, Paul Bassan, Gianfelice Cinque and Peter Gardner
Molecular BioSystems 9 (2013) 677-692
10 Exploring the spectroscopic differences of Caki-2 cells progressing through the cell cycle while proliferating in vitro
M. Jimenez-Hernandez, C. Hughes, P. Bassan, F. Ball, M. D. Brown, N. W. Clarke and P. Gardner
Analyst 138 (2013) 3957-3966
9 Substrate contributions in micro-ATR of thin samples: implications for analysis of cells, tissue and biological fluids
Paul Bassan, Ashwin Sachdeva, Joe Lee and Peter Gardner
Analyst 138 (2013) 4139-4146
8 The inherent problem of transflection-mode infrared spectroscopic microscopy and the ramifications for biomedical single point and imaging applications
Paul Bassan, Joe Lee, Ashwin Sachdeva, Juliana Pissardini, Konrad M. Dorling, John S. Fletcher, Alex Henderson and Peter Gardner
Analyst 138 (2013) 144-157
7 FTIR microscopy of biological cells and tissue: data analysis using resonant Mie scattering (RMieS) EMSC algorithm
Paul Bassan, Ashwin Sachdeva, Achim Kohler, Caryn Hughes, Alex Henderson, Jonathan Boyle, Jonathan H. Shanks, Michael Brown, Noel W. Clarke and Peter Gardner
Analyst 137 (2012) 1370-1377
6 Synchrotron FTIR analysis of drug treated ovarian A2780 cells: an ability to differentiate cell response to different drugs?
Kevin R. Flower, Intisar Khalifa, Paul Bassan, Damien Démoulin, Edward Jackson, Nicholas P. Lockyer, Alan T. McGown, Philip Miles, Lisa Vaccari and Peter Gardner
Analyst 136 (2011) 498-507
5 RMieS-EMSC correction for infrared spectra of biological cells: Extension using full Mie theory and GPU computing
Bassan, P; Kohler, A; Martens, H; Lee, J; Jackson, E; Lockyer, N; Dumas, P; Brown, M; Clarke, N; Gardner, P
Journal of Biophotonics. 3(8-9) (2010) 609-620
4 SR-FTIR spectroscopy of renal epithelial carcinoma side population cells displaying stem cell-like characteristics
Hughes, C; Liew, M; Sachdeva, A; Bassan, P; Dumas, P; Hart, C A; Brown, M D; Clarke, N W; Gardner, P
Analyst. 135(12) (2010) 3133-3141
3 Resonant Mie Scattering (RMieS) correction of infrared spectra from highly scattering biological samples
Bassan, P; Kohler, A; Martens, H; Lee, J; Byrne, H J; Dumas, P; Gazi, E; Brown, M; Clarke, N; Gardner, P
Analyst. 135(2) (2010) 268-277
2 Reflection contributions to the dispersion artefact in FTIR spectra of single biological cells
Bassan, P; Byrne, H J; Lee, J; Bonnier, F; Clarke, C; Dumas, P; Gazi, E; Brown, M D; Clarke, N W; Gardner, P
Analyst. 134(6) (2009) 1171-1175
1 Resonant Mie scattering in infrared spectroscopy of biological materials - understanding the 'dispersion artefact'
Bassan, P; Byrne, H J; Bonnier, F; Lee, J; Dumas, P; Gardner, P
Analyst. 134(8) (2009) 1586-1593

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