caryn.hughes @ manchester.ac.uk
Caryn Hughes obtained a Master of Chemistry MChem with Industrial Experience in 2007, followed by an Analytical Science PhD entitled ‘Development of FTIR for Drug Response Analysis’ at the University of Manchester in 2011. Experimental work included single cell analysis using benchtop and synchrotron radiation sources for FTIR-Microspectroscopy and learning biological techniques such as cell culture, cytotoxicity assays and flow cytometry. Analytical work concentrated on chemometric techniques such as PCA and LDA.
Currently Caryn is jointly appointed as a Postdoctoral Research Associate in spectral pathology between the Genito Urinary Cancer Research Group headed by Professor Noel Clarke at the School of Cancer and Imaging Studies, Paterson Institute for Cancer Research and the Analytical and Medical Vibrational Spectroscopy Group at the Manchester Institute of Biotechnology under Prof. Peter Gardner at University of Manchester. The focus of her research is now tissue based, using imaging FTIR-Microspectroscopy, immuno-histochemistry/fluorescence and supervised classification-based algorithms such as Artificial Neural Networks (ANNs) and Support Vector Machines(SVM).
Dr Kevin Flower, designing novel organometallic anti-cancer compounds.
Areas of experience
- Cell Biology Skills
- Cell culture
- Proliferation/Viability Assays
- Cytotoxicity screening
- FACS cell cycle analysis
- FTIR of biological samples
- Single biological cells
- Cellular monolayers
- Side Population ‘Stem-cell’ analysis
- Synchrotron FTIR, including beamtime at
- Synchrotron Radiation Source (SRS) Daresbury (SRS) – Daresbury, UK
- Synchrotron SOLEIL, France
- Synchrotron Trieste, Italy
Presentations and Workshops
- SPEC 2010, The University of Manchester, June 26- July 1, 2010 (oral presentation)
- 2010 Genitourinary Cancers Symposium, March 5-7, San Francisco, 2010 (poster)
- FTIR Spectroscopy in Microbiological and Medical Diagnostics, Robert Koch-Institute, Berlin, October 15-16, 2009 (poster)
- RSC Analytical Research Forum 2009. 13 – 15 July 2009, Kent, United Kingdom (poster)
- Young Prostate Researchers Symposium, CRUK, Cambridge March 31, 2009 (poster)
- Spectroscopic and Spectrometric Molecular Pathology and Diagnosis, University of Manchester / Robert Koch Institute Forum, Robert Koch Institute, Berlin, March 20, 2009 (oral presentation)
- DASIM Summer School on Synchrotron Infrared Microspectroscopy and Biomedical Applications, ANKA, Karlsruhe, Germany, June 23-27, 2008.
- 4th Annual DASIM Workshop, the Dublin Institute of Technology, Ireland, June 12-13, 2008 (poster)
- 3rd Annual DASIM Workshop, Kraków, Poland, February 7-8, 2008