Exploiting CELLULOSE SYNTHASE (CESA) class-specificity to probe cellulose microfibril biosynthesis

Exploiting CELLULOSE SYNTHASE (CESA) class-specificity to probe cellulose microfibril biosynthesis

Exploiting CELLULOSE SYNTHASE (CESA) class-specificity to probe cellulose microfibril biosynthesis Manoj Kumar, Laxmi Mishra, Paul Carr, Michael Pilling, Peter Gardner, Shawn D. Mansfield and Simon R. Turner Plant Physiology (2018) doi:10.1104/pp.18.00263 Cellulose microfibrils are the basic units of cellulose in plants. The structure of these microfibrils is at least partly determined by the structure of the cellulose synthase complex. In higher plants, this complex is composed of 18 to 24 catalytic subunits known as CELLULOSE SYNTHASE A (CESA) proteins. Three…

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Clinical Applications of Infrared and Raman Spectroscopy: State of Play and Future Challenges

Clinical Applications of Infrared and Raman Spectroscopy: State of Play and Future Challenges

Clinical Applications of Infrared and Raman Spectroscopy: State of Play and Future Challenges Matthew J. Baker, Hugh Byrne, John M. Chalmers, Peter Gardner, Royston Goodacre, Alex Henderson, Sergei Kazarian, Francis L. Martin, Julian Moger, Nick Stone and Josep Sulé-Suso Analyst (2018) Vibrational spectroscopies, based on infrared absorption and/or Raman scattering provide a detailed fingerprint of a material, based on the chemical content. Diagnostic and prognostic tools based on these technologies have the potential to revolutionise our clinical systems leading to improved patient…

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An evaluation of the application of the aperture infrared SNOM technique to biomedical imaging

An evaluation of the application of the aperture infrared SNOM technique to biomedical imaging

An evaluation of the application of the aperture infrared SNOM technique to biomedical imaging J Ingham, M J Pilling, T Craig, M R F Siggel-King, C I Smith, P Gardner, A Varro, D M Pritchard, S D Barrett, D S Martin, P Harrison, P Unsworth, J D Kumar, A Wolski, A Cricenti, M Luce, M Surman, Y M Saveliev and P Weightman Biomedical Physics & Engineering Express 4 (2018) 025011 doi:10.1088/2057-1976/aaa0de A single human oesophageal adenocarcinoma cell (OE33) has been…

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PhD studentship – FTIR, Raman and SIMS Imaging for Lipidomic Analysis

PhD studentship – FTIR, Raman and SIMS Imaging for Lipidomic Analysis

FTIR, Raman and SIMS Imaging for Lipidomic Analysis of Cellular Systems Prof P Gardner, Dr N Lockyer, Dr J Denbigh Deadline 17 November 2017 The role of lipid metabolism in a number of cellular processes including (i) stem cell differentiation, (ii) drugcell interactions and (iii) epithelial/adipocyte cell interactions, is generally poorly understood. For example it has been recently shown that PC3 cells when co-cultured with adipocyte cells, sequestrate omega-6 lipids and their metabolites which subsequently stimulate cell migration and whilst…

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PhD studentship – Secondary effects of gene mutations

PhD studentship – Secondary effects of gene mutations

Probing the secondary effects of Tp53 and BRCA gene mutations upon cellular physiology using advanced analytical techniques. Prof R Edmondson, Prof P Gardner, Dr N Lockyer, Dr J Denbigh Deadline: 17 November 2017 Cancer is a disease of DNA in which genomic events allow the cell to develop the autonomy, increased proliferation and other fundamental hallmarks of the disease. This process is often initiated by mutation of one or two key driver genes. Understanding the effects of these driver mutations…

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Quantum Cascade Laser Spectral Histopathology: Breast Cancer Diagnostics Using High Throughput Chemical Imaging

Quantum Cascade Laser Spectral Histopathology: Breast Cancer Diagnostics Using High Throughput Chemical Imaging

Quantum Cascade Laser Spectral Histopathology: Breast Cancer Diagnostics Using High Throughput Chemical Imaging Michael J. Pilling, Alex Henderson and Peter Gardner Analytical Chemistry 89(14) (2017) 7348–7355 doi:10.1021/acs.analchem.7b00426 Fourier transform infrared (FT-IR) microscopy coupled with machine learning approaches has been demonstrated to be a powerful technique for identifying abnormalities in human tissue. The ability to objectively identify the prediseased state and diagnose cancer with high levels of accuracy has the potential to revolutionize current histopathological practice. Despite recent technological advances in…

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CLIRCON17

CLIRCON17

    The CLIRCON17 Conference will be held in Manchester, UK, 2–5 April 2017. It is hosted by the Clinical Infrared and Raman Spectroscopy (CLIRSPEC) Network and the Analytical and Biomedical Spectroscopy groups at the University of Manchester, UK. The conference is for researchers and industrialists in the multidisciplinary area of clinical infrared and Raman spectroscopy. For more information, please visit: https://clirspec.org/uk-network/clircon17/

Paper: Infrared spectral histopathology using haematoxylin and eosin (H&E) stained glass slides: a major step forward towards clinical translation

Paper: Infrared spectral histopathology using haematoxylin and eosin (H&E) stained glass slides: a major step forward towards clinical translation

Infrared spectral histopathology using haematoxylin and eosin (H&E) stained glass slides: a major step forward towards clinical translation Michael J. Pilling, Alex Henderson, Jonathan H. Shanks, Michael D. Brown, Noel W. Clarke and Peter Gardner Analyst 142 (2017) 1258-1268 doi: 10.1039/C6AN02224C Infrared spectral histopathology has shown great promise as an important diagnostic tool, with the potential to complement current pathological methods. While promising, clinical translation has been hindered by the impracticalities of using infrared transmissive substrates which are both fragile…

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Paper – FTIR imaging of the molecular burden around Aβ deposits in an early-stage 3-Tg-APP-PSP1-TAU mouse model of Alzheimer’s disease

Paper – FTIR imaging of the molecular burden around Aβ deposits in an early-stage 3-Tg-APP-PSP1-TAU mouse model of Alzheimer’s disease

FTIR imaging of the molecular burden around Aβ deposits in an early-stage 3-Tg-APP-PSP1-TAU mouse model of Alzheimer’s disease Artur Dawid Surowka, Michael Pilling, Alex Henderson, Herve Boutin, Lidan Christie, Magdalena Szczerbowska-Boruchowska and Peter Gardner Analyst 142 (2017) 156-168 doi:10.1039/C6AN01797E Alzheimer’s disease is one of the major causes of dementia in the elderly. The disease is caused by the misfolding of water soluble alpha-helical proteins, which leads to the accumulation of β-sheets in the form of amyloid plaques, which can subsequently…

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Review Paper – Single cell analysis using Fourier transform infrared microspectroscopy

Review Paper – Single cell analysis using Fourier transform infrared microspectroscopy

Single Cell Analysis sing Fourier Transform Infrared Microspectroscopy James Doherty, Gianfelice Cinque & Peter Gardner Applied Spectroscopy Reviews(Oct 2016) Abstract Fourier transform infrared spectroscopy (FTIR) is a well-established, non-destructive method of obtaining chemical information from biological samples such as tissues and cells. This review focuses specifically on the development of infrared spectroscopic microanalysis at the single cell level. Technological developments, including that of the infrared microscope, synchrotron radiation (SR)-FTIR, and focal plane array (FPA) detectors, and their impact on the…

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