Peter Gardner's Lab

Exploiting CELLULOSE SYNTHASE (CESA) class-specificity to probe cellulose microfibril biosynthesis Manoj Kumar, Laxmi Mishra, Paul Carr, Michael Pilling, Peter Gardner, Shawn D. Mansfield and Simon R. Turner Plant Physiology (2018) doi:10.1104/pp.18.00263 Cellulose microfibrils are the basic units of cellulose in plants. The structure of these microfibrils is at least partly determined by the structure of the cellulose synthase complex. In higher plants, this complex is composed of 18 to 24 catalytic subunits known as CELLULOSE SYNTHASE A (CESA) proteins. Three…

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Clinical Applications of Infrared and Raman Spectroscopy: State of Play and Future Challenges Matthew J. Baker, Hugh Byrne, John M. Chalmers, Peter Gardner, Royston Goodacre, Alex Henderson, Sergei Kazarian, Francis L. Martin, Julian Moger, Nick Stone and Josep Sulé-Suso Analyst (2018) Vibrational spectroscopies, based on infrared absorption and/or Raman scattering provide a detailed fingerprint of a material, based on the chemical content. Diagnostic and prognostic tools based on these technologies have the potential to revolutionise our clinical systems leading to improved patient…

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An evaluation of the application of the aperture infrared SNOM technique to biomedical imaging J Ingham, M J Pilling, T Craig, M R F Siggel-King, C I Smith, P Gardner, A Varro, D M Pritchard, S D Barrett, D S Martin, P Harrison, P Unsworth, J D Kumar, A Wolski, A Cricenti, M Luce, M Surman, Y M Saveliev and P Weightman Biomedical Physics & Engineering Express 4 (2018) 025011 doi:10.1088/2057-1976/aaa0de A single human oesophageal adenocarcinoma cell (OE33) has been…

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Quantum Cascade Laser Spectral Histopathology: Breast Cancer Diagnostics Using High Throughput Chemical Imaging Michael J. Pilling, Alex Henderson and Peter Gardner Analytical Chemistry 89(14) (2017) 7348–7355 doi:10.1021/acs.analchem.7b00426 Fourier transform infrared (FT-IR) microscopy coupled with machine learning approaches has been demonstrated to be a powerful technique for identifying abnormalities in human tissue. The ability to objectively identify the prediseased state and diagnose cancer with high levels of accuracy has the potential to revolutionize current histopathological practice. Despite recent technological advances in…

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Infrared spectral histopathology using haematoxylin and eosin (H&E) stained glass slides: a major step forward towards clinical translation Michael J. Pilling, Alex Henderson, Jonathan H. Shanks, Michael D. Brown, Noel W. Clarke and Peter Gardner Analyst 142 (2017) 1258-1268 doi: 10.1039/C6AN02224C Infrared spectral histopathology has shown great promise as an important diagnostic tool, with the potential to complement current pathological methods. While promising, clinical translation has been hindered by the impracticalities of using infrared transmissive substrates which are both fragile…

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FTIR imaging of the molecular burden around Aβ deposits in an early-stage 3-Tg-APP-PSP1-TAU mouse model of Alzheimer’s disease Artur Dawid Surowka, Michael Pilling, Alex Henderson, Herve Boutin, Lidan Christie, Magdalena Szczerbowska-Boruchowska and Peter Gardner Analyst 142 (2017) 156-168 doi:10.1039/C6AN01797E Alzheimer’s disease is one of the major causes of dementia in the elderly. The disease is caused by the misfolding of water soluble alpha-helical proteins, which leads to the accumulation of β-sheets in the form of amyloid plaques, which can subsequently…

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Single Cell Analysis sing Fourier Transform Infrared Microspectroscopy James Doherty, Gianfelice Cinque & Peter Gardner Applied Spectroscopy Reviews(Oct 2016) Abstract Fourier transform infrared spectroscopy (FTIR) is a well-established, non-destructive method of obtaining chemical information from biological samples such as tissues and cells. This review focuses specifically on the development of infrared spectroscopic microanalysis at the single cell level. Technological developments, including that of the infrared microscope, synchrotron radiation (SR)-FTIR, and focal plane array (FPA) detectors, and their impact on the…

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The Dynamic Nature of Hypertrophic and Fibrotic Remodeling of the Fish Ventricle A. N. Keen, A. J. Fenna,  J. C. McConnell,  M. J. Sherratt, P. Gardner, H. A. Shiels Frontiers in Physiology 6 (2016) 427 doi: 10.3389/fphys.2015.00427 Abstract Chronic pressure or volume overload can cause the vertebrate heart to remodel. The hearts of fish remodel in response to seasonal temperature change. Here we focus on the passive properties of the fish heart. Building upon our previous work on thermal-remodeling of the rainbow trout ventricle, we…

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Fundamental developments in infrared spectroscopic imaging for biomedical applications Michael Pilling and Peter Gardner Chem. Soc. Rev., 2016,45, 1935-1957 DOI: 10.1039/C5CS00846H Abstract Infrared chemical imaging is a rapidly emerging field with new advances in instrumentation, data acquisition and data analysis. These developments have had significant impact in biomedical applications and numerous studies have now shown that this technology offers great promise for the improved diagnosis of the diseased state. Relying on purely biochemical signatures rather than contrast from exogenous dyes and stains, infrared…

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