Peter Gardner's Lab

Chemotherapeutic response to cisplatin-like drugs in human breast cancer cells probed by vibrational microspectroscopy A. L. M. Batista de Carvalho, M. Pilling, P. Gardner, J. Doherty, G. Cinque, K. Wehbe, C. Kelley, L. A. E. Batista de Carvalho and   M. P. M. Marques Faraday Discuss., 2016,187, 273-298 DOI: 10.1039/C5FD00148J Abstract Studies of drug–cell interactions in cancer model systems are essential in the preclinical stage of rational drug design, which relies on a thorough understanding of the mechanisms underlying cytotoxic activity and biological effects, at a molecular level….

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High-throughput quantum cascade laser (QCL) spectral histopathology: a practical approach towards clinical translation M. J. Pilling, A. Henderson, B. Bird,  M. D. Brown, N. W. Clarke and P. Gardner Faraday Discuss., 2016,187, 135-154 DOI: 10.1039/C5FD00176E Abstract Infrared microscopy has become one of the key techniques in the biomedical research field for interrogating tissue. In partnership with multivariate analysis and machine learning techniques, it has become widely accepted as a method that can distinguish between normal and cancerous tissue with both high sensitivity and high specificity. While…

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Automated analysis of single cells using Laser Tweezers Raman Spectroscopy S. Casabella, P. Scully, N. Goddard and P. Gardner Analyst, 2016, Advance Article DOI: 10.1039/C5AN01851J

Characterising cytotoxic agent action as a function of the cell cycle using fourier transform infrared microspectroscopy M. Jimenez-Hernandez, M.D. Brown, C. Hughes, N.W. Clarke and P. Gardner Analyst (2015)

Enhanced FTIR benchtop imaging of single biological cells Caryn Hughes, Alex Henderson, Mustafa Kansiz, Konrad Dorling, Melody Jimenez-Hernandez, Michael D Brown, Noel William Clarke and Peter Gardner Analyst 140 (2015) 2080-2085

Comparison of transmission and transflectance mode FTIR imaging of biological tissue Michael J. Pilling, Paul Bassan and Peter Gardner Analyst (2015)

Using Fourier transform IR spectroscopy to analyze biological materials Matthew J Baker, Júlio Trevisan, Paul Bassan, Rohit Bhargava, Holly J Butler, Konrad M Dorling, Peter R Fielden, Simon W Fogarty, Nigel J Fullwood, Kelly A Heys, Caryn Hughes, Peter Lasch, Pierre L Martin-Hirsch, Blessing Obinaju, Ganesh D Sockalingum, Josep Sulé-Suso, Rebecca J Strong, Michael J Walsh, Bayden R Wood, Peter Gardner & Francis L Martin Nature Protocols 9 (2014) 1771–1791

Large scale infrared imaging of tissue micro arrays (TMAs) using a tunable Quantum Cascade Laser (QCL) based microscope Paul Bassan, Miles J. Weida, Jeremy Rowlette and Peter Gardner Analyst (2014) Abstract Chemical imaging in the field of vibrational spectroscopy is developing into a promising tool to complement digital histopathology. Applications include screening of biopsy tissue via automated recognition of tissue/cell type and disease state based on the chemical information from the spectrum. For integration into clinical practice, data acquisition needs…

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Automated high-throughput assessment of prostate biopsy tissue using infrared spectroscopic chemical imaging Paul Bassan, Ashwin Sachdeva, Jonathan H. Shanks, Mick D. Brown, Noel W. Clarke and Peter Gardner Proc. SPIE 9041, Medical Imaging 2014: Digital Pathology, 90410D 9041 (2014) Abstract Fourier transform infrared (FT-IR) chemical imaging has been demonstrated as a promising technique to complement histopathological assessment of biomedical tissue samples. Current histopathology practice involves preparing thin tissue sections and staining them using hematoxylin and eosin (H&E) after which a…

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Assessing the challenges of Fourier transform infrared spectroscopic analysis of blood serum Caryn Hughes, Michael Brown, Graeme Clemens, Alex Henderson, Geraldine Monjardez, Noel W. Clarke and Peter Gardner Journal of Biophotonics (2014) [OPEN ACCESS] Abstract There are many approaches to measuring the infrared spectrum of a blood serum sample. Naturally, each approach will have both advantages and disadvantages. We report on the progress of the application of infrared spectroscopy in the field of blood serum analysis towards clinical application, with…

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