Peter Gardner's Lab

Assessment of paraffin removal from prostate FFPE sections using transmission mode FTIR-FPA imaging Caryn Hughes, Lydia Gaunt, Michael Brown, Noel W. Clarke and Peter Gardner Anal. Methods (2014) Abstract Formalin-fixed paraffin-embedded (FFPE) tissue sections are routinely analysed for biochemical discrimination by vibrational spectroscopy techniques in the spectral pathology community. In these experiments, it is usually desirable to remove the paraffin from the tissue. This is most commonly performed by the use of xylene but other solvents such as hexane are…

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Transmission FTIR chemical imaging on glass substrates: applications in infrared spectral histopathology Paul Bassan, Joe Mellor, Jonathan Shapiro, Kaye J. Williams, Michael Lisanti and Peter Gardner Anal. Chem. (2014) Abstract Fourier transform infrared chemical imaging in transmission mode has traditionally been performed on expensive mid-IR transparent windows such as barium/calcium fluoride, which are more fragile than glass making preparation in the histopathology laboratories more cumbersome. A solution is presented here by using cheap glass substrates for the FTIR chemical imaging…

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Whole organ cross-section chemical imaging using label-free mega-mosaic FTIR microscopy Paul Bassan, Ashwin Sachdeva, Jonathan H. Shanks, Mick D. Brown, Noel W. Clarke and Peter Gardner Analyst (2013) Abstract FTIR chemical imaging has been demonstrated as a promising technique to construct automated systems to complement histopathological evaluation of biomedical tissue samples. The rapid chemical imaging of large areas of tissue has previously been a limiting factor in this application. Consequently, smaller areas of tissue have previously had to be sampled,…

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FTIR microspectroscopy of selected rare diverse sub-variants of carcinoma of the urinary bladder Caryn Hughes, Junaid Iqbal-Wahid, Michael Brown, Jonathan H. Shanks, Amanda Eustace, Helen Denley, Peter J. Hoskin, Catharine West, Noel W. Clarke, Peter Gardner Journal of Biophotonics (2012) Abstract Urothelial carcinomas of the bladder are a heterogeneous group of tumours, although some histological sub-variants are rare and sparsely reported in the literature. Diagnosis of sub-variants from conventional urothelial carcinoma can be challenging, as they may mimic the morphology…

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The inherent problem of transflection-mode infrared spectroscopic microscopy and the ramifications for biomedical single point and imaging applications Paul Bassan, Joe Lee, Ashwin Sachdeva, Juliana Pissardini, Konrad M. Dorling, John S. Fletcher, Alex Henderson and Peter Gardner Analyst, 2013, 138, 144-157 Abstract Transflection-mode FTIR spectroscopy has become a popular method of measuring spectra from biomedical and other samples due to the relative low cost of substrates compared to transmission windows, and a higher absorbance due to a double pass through…

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Highlighting a need to distinguish cell cycle signatures from cellular responses to chemotherapeutics in SR-FTIR spectroscopy C. Hughes, M. D. Brown, F. J. Ball, G. Monjardez, N. W. Clarke, K. R. Flower and P. Gardner Analyst 137 (2012) 5736-5742 Abstract Previous research has seen difficulties in establishing clear discrimination by principal component analysis (PCA) between drug-treated cells analysed by single point SR-FTIR spectroscopy, relative to multisampling cell monolayers by conventional FTIR. It is suggested that the issue arises due to…

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Investigating cellular responses to novel chemotherapeutics in renal cell carcinoma using SR-FTIR spectroscopy C. Hughes, M. D. Brown, N. W. Clarke, K. R. Flower and P. Gardner Analyst 137 (2012) 4720-4726 Abstract SR-FTIR spectroscopy was evaluated as a technique to discriminate spectral signals of cellular response at the single cell level, when cancer cells are exposed to chemotherapeutics. 5-Fluorouracil, an established drug of known mode of action, was tested against a renal carcinoma cell line (Caki-2), along with two experimental…

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Spectroscopy: Infrared and Raman spectroscopy in biomedical research, Innovation into Success P. Gardner The Quarterly Journal of UKSPA 29 (2012) 23