Research Associate paul.bassan @ manchester.ac.uk
PhD on Biomedical Fourier transform infared (FTIR) spectroscopy of highly scattering samples: Understanding and correcting resonant Mie scattering (RMieS), University of Manchester, UK. 2011 MSc in Medical Physics, University of Manchester, UK. 2007 BSc Physics, University of Warwick, UK. 2006 CEO, London Spectroscopy Ltd.

Paul Bassan received his B.Sc. in Physics at the University of Warwick U.K. in 2006 and then went on to receive an M.Sc. in Medical Physics at the University of Manchester U.K. in 2007. In October 2007 he joined the Gardner group at the University of Manchester and is currently researching the fundamental principles of mid-IR light scattering (namely Mie scattering) in single cell and tissue samples as used in FTIR spectroscopy. Eventual aims are to produce a correction algorithm which recovers the pure absorbance spectra of highly scattering samples. A preliminary version called the resonant Mie scattering extended multiplicative signal correction (RMieS-EMSC) has been written (in collaboration with Achim Kohler and Harald Martens of Nofima Mat, Norway) and distributed free of charge to academic users. In the future he plans to apply the RMieS-EMSC to FTIR imaging data with the use of parallel computing using graphics processing unit (GPU) computing.

Areas of experience

• Matlabprogramming
  • Writing algorithms
  • GPU Processing

• FTIR of highly scattering samples
  • Single biological cells
  • Resonant Mie scattering (RMieS-EMSC)

• Synchrotron FTIR, including beamtime at
  • Synchrotron Radiation Source (SRS) Daresbury (SRS) – Daresbury, UK
  • Synchrotron SOLEIL, France
  • Diamond Light Source, Oxford, UK

Books

1. Paul Bassan, and Peter Gardner, Scattering in Biomedical Infrared Spectroscopy, in Biomedical Applications of Synchrotron Infrared Microspectroscopy, The Royal Society of Chemistry. p. 260-276.

Publications

17	Comparison of transmission and transflectance mode FTIR imaging of biological tissue Michael J. Pilling, Paul Bassan and Peter Gardner Analyst 140 (2015) 2383-2392
16	Using Fourier transform IR spectroscopy to analyze biological materials Matthew J Baker, Júlio Trevisan, Paul Bassan, Rohit Bhargava, Holly J Butler, Konrad M Dorling, Peter R Fielden, Simon W Fogarty, Nigel J Fullwood, Kelly A Heys, Caryn Hughes, Peter Lasch, Pierre L Martin-Hirsch, Blessing Obinaju, Ganesh D Sockalingum, Josep Sulé-Suso, Rebecca J Strong, Michael J Walsh, Bayden R Wood, Peter Gardner & Francis L Martin Nature Protocols 9 (2014) 1771–1791
15	Large scale infrared imaging of tissue micro arrays (TMAs) using a tunable Quantum Cascade Laser (QCL) based microscope Paul Bassan, Miles J. Weida, Jeremy Rowlette and Peter Gardner Analyst 139 (2014) 3856-3859
14	Automated high-throughput assessment of prostate biopsy tissue using infrared spectroscopic chemical imaging Paul Bassan, Ashwin Sachdeva, Jonathan H. Shanks, Mick D. Brown, Noel W. Clarke and Peter Gardner Proc. SPIE 9041, Medical Imaging 2014: Digital Pathology 9041 (2014) 90410D-90410D-10
13	Transmission FTIR chemical imaging on glass substrates: applications in infrared spectral histopathology Paul Bassan, Joe Mellor, Jonathan Shapiro, Kaye J. Williams, Michael Lisanti and Peter Gardner Anal. Chem. 86(3) (2014) 1648–1653
12	Whole organ cross-section chemical imaging using label-free mega-mosaic FTIR microscopy Paul Bassan, Ashwin Sachdeva, Jonathan H. Shanks, Mick D. Brown, Noel W. Clarke and Peter Gardner Analyst 138 (2013) 7066-7069
11	The action of all-trans-retinoic acid (ATRA) and synthetic retinoid analogues (EC19 and EC23) on human pluripotent stem cells differentiation investigated using single cell infrared microspectroscopy. Graeme Clemens, Kevin R. Flower, Andrew P. Henderson, Andrew Whiting, Stefan A. Przyborski, Melody Jimenez-Hernandez, Francis Ball, Paul Bassan, Gianfelice Cinque and Peter Gardner Molecular BioSystems 9 (2013) 677-692
10	Exploring the spectroscopic differences of Caki-2 cells progressing through the cell cycle while proliferating in vitro M. Jimenez-Hernandez, C. Hughes, P. Bassan, F. Ball, M. D. Brown, N. W. Clarke and P. Gardner Analyst 138 (2013) 3957-3966
9	Substrate contributions in micro-ATR of thin samples: implications for analysis of cells, tissue and biological fluids Paul Bassan, Ashwin Sachdeva, Joe Lee and Peter Gardner Analyst 138 (2013) 4139-4146
8	The inherent problem of transflection-mode infrared spectroscopic microscopy and the ramifications for biomedical single point and imaging applications Paul Bassan, Joe Lee, Ashwin Sachdeva, Juliana Pissardini, Konrad M. Dorling, John S. Fletcher, Alex Henderson and Peter Gardner Analyst 138 (2013) 144-157
7	FTIR microscopy of biological cells and tissue: data analysis using resonant Mie scattering (RMieS) EMSC algorithm Paul Bassan, Ashwin Sachdeva, Achim Kohler, Caryn Hughes, Alex Henderson, Jonathan Boyle, Jonathan H. Shanks, Michael Brown, Noel W. Clarke and Peter Gardner Analyst 137 (2012) 1370-1377
6	Synchrotron FTIR analysis of drug treated ovarian A2780 cells: an ability to differentiate cell response to different drugs? Kevin R. Flower, Intisar Khalifa, Paul Bassan, Damien Démoulin, Edward Jackson, Nicholas P. Lockyer, Alan T. McGown, Philip Miles, Lisa Vaccari and Peter Gardner Analyst 136 (2011) 498-507
5	RMieS-EMSC correction for infrared spectra of biological cells: Extension using full Mie theory and GPU computing Bassan, P; Kohler, A; Martens, H; Lee, J; Jackson, E; Lockyer, N; Dumas, P; Brown, M; Clarke, N; Gardner, P Journal of Biophotonics 3(8-9) (2010) 609-620
4	SR-FTIR spectroscopy of renal epithelial carcinoma side population cells displaying stem cell-like characteristics Hughes, C; Liew, M; Sachdeva, A; Bassan, P; Dumas, P; Hart, C A; Brown, M D; Clarke, N W; Gardner, P Analyst 135(12) (2010) 3133-3141
3	Resonant Mie Scattering (RMieS) correction of infrared spectra from highly scattering biological samples Bassan, P; Kohler, A; Martens, H; Lee, J; Byrne, H J; Dumas, P; Gazi, E; Brown, M; Clarke, N; Gardner, P Analyst 135(2) (2010) 268-277
2	Reflection contributions to the dispersion artefact in FTIR spectra of single biological cells Bassan, P; Byrne, H J; Lee, J; Bonnier, F; Clarke, C; Dumas, P; Gazi, E; Brown, M D; Clarke, N W; Gardner, P Analyst 134(6) (2009) 1171-1175
1	Resonant Mie scattering in infrared spectroscopy of biological materials - understanding the 'dispersion artefact' Bassan, P; Byrne, H J; Bonnier, F; Lee, J; Dumas, P; Gardner, P Analyst 134(8) (2009) 1586-1593

Other links

• Paul Bassan’s SpecMod profile
• Nofima Mat, Norway
• University of Manchester
• Manchester Institute of Biotechnology (MIB) 
• Paterson Institute for Cancer Research
• University of Warwick