Using Fourier transform IR spectroscopy to analyze biological materials Matthew J Baker, Júlio Trevisan, Paul Bassan, Rohit Bhargava, Holly J Butler, Konrad M Dorling, Peter R Fielden, Simon W Fogarty, Nigel J Fullwood, Kelly A Heys, Caryn Hughes, Peter Lasch, Pierre L Martin-Hirsch, Blessing Obinaju, Ganesh D Sockalingum, Josep Sulé-Suso, Rebecca J Strong, Michael J Walsh, Bayden R Wood, Peter Gardner & Francis L Martin Nature Protocols 9 (2014) 1771–1791
Large scale infrared imaging of tissue micro arrays (TMAs) using a tunable Quantum Cascade Laser (QCL) based microscope Paul Bassan, Miles J. Weida, Jeremy Rowlette and Peter Gardner Analyst (2014) Abstract Chemical imaging in the field of vibrational spectroscopy is developing into a promising tool to complement digital histopathology. Applications include screening of biopsy tissue via automated recognition of tissue/cell type and disease state based on the chemical information from the spectrum. For integration into clinical practice, data acquisition needs…
Automated high-throughput assessment of prostate biopsy tissue using infrared spectroscopic chemical imaging Paul Bassan, Ashwin Sachdeva, Jonathan H. Shanks, Mick D. Brown, Noel W. Clarke and Peter Gardner Proc. SPIE 9041, Medical Imaging 2014: Digital Pathology, 90410D 9041 (2014) Abstract Fourier transform infrared (FT-IR) chemical imaging has been demonstrated as a promising technique to complement histopathological assessment of biomedical tissue samples. Current histopathology practice involves preparing thin tissue sections and staining them using hematoxylin and eosin (H&E) after which a…
Assessing the challenges of Fourier transform infrared spectroscopic analysis of blood serum Caryn Hughes, Michael Brown, Graeme Clemens, Alex Henderson, Geraldine Monjardez, Noel W. Clarke and Peter Gardner Journal of Biophotonics (2014) [OPEN ACCESS] Abstract There are many approaches to measuring the infrared spectrum of a blood serum sample. Naturally, each approach will have both advantages and disadvantages. We report on the progress of the application of infrared spectroscopy in the field of blood serum analysis towards clinical application, with…
Assessment of paraffin removal from prostate FFPE sections using transmission mode FTIR-FPA imaging Caryn Hughes, Lydia Gaunt, Michael Brown, Noel W. Clarke and Peter Gardner Anal. Methods (2014) Abstract Formalin-fixed paraffin-embedded (FFPE) tissue sections are routinely analysed for biochemical discrimination by vibrational spectroscopy techniques in the spectral pathology community. In these experiments, it is usually desirable to remove the paraffin from the tissue. This is most commonly performed by the use of xylene but other solvents such as hexane are…
Transmission FTIR chemical imaging on glass substrates: applications in infrared spectral histopathology Paul Bassan, Joe Mellor, Jonathan Shapiro, Kaye J. Williams, Michael Lisanti and Peter Gardner Anal. Chem. (2014) Abstract Fourier transform infrared chemical imaging in transmission mode has traditionally been performed on expensive mid-IR transparent windows such as barium/calcium fluoride, which are more fragile than glass making preparation in the histopathology laboratories more cumbersome. A solution is presented here by using cheap glass substrates for the FTIR chemical imaging…
Whole organ cross-section chemical imaging using label-free mega-mosaic FTIR microscopy Paul Bassan, Ashwin Sachdeva, Jonathan H. Shanks, Mick D. Brown, Noel W. Clarke and Peter Gardner Analyst (2013) Abstract FTIR chemical imaging has been demonstrated as a promising technique to construct automated systems to complement histopathological evaluation of biomedical tissue samples. The rapid chemical imaging of large areas of tissue has previously been a limiting factor in this application. Consequently, smaller areas of tissue have previously had to be sampled,…
Today we welcome a new student – Julie Brooks. Julie will be working on a joint project with Dr Nick Lockyer in the School of Chemistry and Surface Analysis Research Centre developing infrared spectroscopy techniques for the clinical diagnosis of prostate cancer in blood serum.
SPEC 2014, Shedding new Light on Disease will be held in Krakow, Poland, 17-22 August 2014. For more information visit the official website of the conference: SPEC 2014
The Gardner group made an impact on the SPEC 2012 conference in Chiang Mai with five oral presentations and three posters. The presentations were well received, although Peter may have disappointed some of the audience by showing how their transflection mode data is seriously flawed (see our paper on the subject).