Peter Gardner's Lab

FTIR microspectroscopy of selected rare diverse sub-variants of carcinoma of the urinary bladder Caryn Hughes, Junaid Iqbal-Wahid, Michael Brown, Jonathan H. Shanks, Amanda Eustace, Helen Denley, Peter J. Hoskin, Catharine West, Noel W. Clarke, Peter Gardner Journal of Biophotonics (2012) Abstract Urothelial carcinomas of the bladder are a heterogeneous group of tumours, although some histological sub-variants are rare and sparsely reported in the literature. Diagnosis of sub-variants from conventional urothelial carcinoma can be challenging, as they may mimic the morphology…

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The inherent problem of transflection-mode infrared spectroscopic microscopy and the ramifications for biomedical single point and imaging applications Paul Bassan, Joe Lee, Ashwin Sachdeva, Juliana Pissardini, Konrad M. Dorling, John S. Fletcher, Alex Henderson and Peter Gardner Analyst, 2013, 138, 144-157 Abstract Transflection-mode FTIR spectroscopy has become a popular method of measuring spectra from biomedical and other samples due to the relative low cost of substrates compared to transmission windows, and a higher absorbance due to a double pass through…

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Highlighting a need to distinguish cell cycle signatures from cellular responses to chemotherapeutics in SR-FTIR spectroscopy C. Hughes, M. D. Brown, F. J. Ball, G. Monjardez, N. W. Clarke, K. R. Flower and P. Gardner Analyst 137 (2012) 5736-5742 Abstract Previous research has seen difficulties in establishing clear discrimination by principal component analysis (PCA) between drug-treated cells analysed by single point SR-FTIR spectroscopy, relative to multisampling cell monolayers by conventional FTIR. It is suggested that the issue arises due to…

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Investigating cellular responses to novel chemotherapeutics in renal cell carcinoma using SR-FTIR spectroscopy C. Hughes, M. D. Brown, N. W. Clarke, K. R. Flower and P. Gardner Analyst 137 (2012) 4720-4726 Abstract SR-FTIR spectroscopy was evaluated as a technique to discriminate spectral signals of cellular response at the single cell level, when cancer cells are exposed to chemotherapeutics. 5-Fluorouracil, an established drug of known mode of action, was tested against a renal carcinoma cell line (Caki-2), along with two experimental…

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Spectroscopy: Infrared and Raman spectroscopy in biomedical research, Innovation into Success P. Gardner The Quarterly Journal of UKSPA 29 (2012) 23

FTIR microscopy of biological cells and tissue: data analysis using resonant Mie scattering (RMieS) EMSC algorithm Paul Bassan, Ashwin Sachdeva, Achim Kohler, Caryn Hughes, Alex Henderson, Jonathan Boyle, Jonathan H. Shanks, Michael Brown, Noel W. Clarke and Peter Gardner Analyst 137 (2012) 1370-1377 Abstract Transmission and transflection infrared microscopy of biological cells and tissue suffer from significant baseline distortions due to scattering effects, predominantly resonant Mie scattering (RMieS). This scattering can also distort peak shapes and apparent peak positions making…

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